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Delocalized long-lived proton spin states in aliphatic chains
Kirill Sheberstov, Ecole Normale Superiuer, France Summary Long-lived states (LLS) have lifetimes T_{LLS} that can be much longer than longitudinal relaxation times T_1. However, the number of molecules where such states can be excited has hitherto been rather limited. In the talk, it will be discussed that LLS can be readily excited in many common molecules…
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Three new tricks on established solid-state NMR pulse sequences for structure determination
Xue Kai, Nanyang Technological University, Singapore Summary Solid state NMR is widely used for atomic level structural investigations. Different pulse sequences are designed for specific systems and information. Here we list three new tricks on the well-established xHHx, hetCOR, and CODEX pulse sequences. These new tricks enable the more efficient and accurate extraction of structural…
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Hyperpolarization Techniques in NMR: Applications to Biological Systems
James Eills, Institute for Bioengineering of Catalonia (Spain) Hyperpolarization techniques are methods to enhance NMR signals beyond those achievable at thermal equilibrium, and they have had an impact on almost every sub-field in NMR. In this talk I will give an overview of available hyperpolarization techniques and how they are related, and discuss some interesting cases…
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Watson-Crick-like tautomeric and anionic G-U/T conformational states in RNA-DNA hybrids: Context dependence and implications for transcriptional errors
Or Szekely, Duke University (USA) Nucleic acid G•T/U wobble mismatches can transiently form low-abundance Watson-Crick (WC) like conformations through tautomerization or ionization of the bases. These conformational dynamics have been observed in B-DNA and A-RNA duplexes and are implicated in the mechanisms of replication and translation errors. However, it is not known whether these dynamics…
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19F Solid-State NMR Application in PET Imaging Agent Binding and Protein Structural Assignment
Pu Duan, Massachusetts Institute of Technology (USA) Fluorinated small molecules and fluorinated proteins are ubiquitous in the pharmaceutical industry, medical imaging, and structural biological research, and the presence of fluorine atoms provides the opportunity to employ 19F solid-state NMR. Here, we demonstrate the power of 19F solid-state NMR by studying the binding sites of Flutemetamol, a…
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Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function
Benjamin Bourgeois, Med Uni Graz, Austria Summary
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Using pulsed EPR and spin labelling to investigate the conformational landscape of integral membrane proteins in lipid bilayers
Christos Pilotas, University of Leeds, UK Summary
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The role of conformational entropy in molecular evolution of bacterial transcriptional repressors
Daiana Capdevila, Leloir Insitute, Argentina Summary
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Competing stress-dependent oligomerization pathways regulate self-assembly of the periplasmic protease-chaperone DegP
Rob Harkness, University of Toronto, Canada Summary
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