Summary

Amelotin plays a crucial role in the hydroxyapatite mineralization of dental enamel. Due to their dynamic and heterogeneous nature, large oligomeric species formed by Amelotin are not amenable to structural studies at the residue-specific level. Here, we present new pressure-jump NMR methods for probing the structure of oligomeric species. Secondary and tertiary structure information on the oligomers obtained from ¹³C chemical shifts and ¹H-¹H nuclear Overhauser enhancements (NOE) will be demonstrated.